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KMID : 0364819760140020075
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Korean Journal of Microbiology
1976 Volume.14 No. 2 p.75 ~ p.83
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Influence of some metal ions on the cellulase activity
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Choi Myung-Ja
Kim Young-MIn
Kim Eun-Soo
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Abstract
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Three commercial cellulases prepared from Penicillium notatum(cellulalse[K]), Trichoderma viride(cellulase[J]) and Aspergillus niger(cellulase[A]) were analyzed with respect to their relative purity, activity and the effects of several metal ions on their activities. The activity of cellulase[K] was the strongest of all and that of cellulase[A] being the weaker. The purity of cellulalse[K] was the highest while that of cellulase[J] was the lowest. Under the assay conditions, the optimum concentrations of Zn^++ and Mg^++ ions for the activity of cellulase[K] was the highest while that of cellulase[J] was the lowest. Under the assay conditions, the optimum concentrations of Zn^++ and Mg^++ ions for the activity of cellulalse[K] was the highest while that of cellulase [A] being weaker. The purity of cellulase[K] was the highest while that of cellulase[J] was the lowest. Under the assay conditions, the optimum concentrations of Zn^++ and Mg^++ ions for the activity of cellulase[K] were 2 and 7mM while those of cellulase[A] were 5 and 6 mM respectively and those of cellulase[J] were 3mM for both ions. Cellulase[K] and cellulase[J] were more strongly activated by Zn^++ than Mg^++ and cellulase[J] by Mg^++ than Zn^++. Both Cu^++ and Mn^++ ions inhibited by these metal ions the inhibitory effects of Mn^++ ions for enzyme activities were stronger than Cu^++ ions. The Ki values of Cu^++ and Mn^++ for cellulase[K] were found to be 6.1 and 0.7mM, those of cellulase[J] were 2.6 and 0.32 mM, and those of cellulalse[A] were 2.0 and 0.2 mM respectively. Both Cu^++ and Mn^++ ions showed a pattern of competitive inhibition of the enzyme activity. When Na-CMC was used as substrate, the Km and V values of celluase [K] were calculated to be 2.0 × 10^-4 mM and 3.43mmoles/hour, those of cellulase[J] were 2.5 × 10^-4 mM and 3.77mmoles/hour, and those of cellulase[A] were 4.0 × 10 ^-4 mM and 4.00mmoles/hour respectively.
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KEYWORD
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Influence of some metal ions on the cellulase activity
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